LOCUS CG31813_gDNA_CRI 5275 bp ds-DNA linear 14-MAY-2016 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT CG31813 gDNA COMMENT SacI(HT31/HT26=112+1584=1696bp) COMMENT MaeI(OMS317/HT26=281+1858=2139bp) COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 2001..2050 /label=attp site /ApEinfo_fwdcolor=#ff00ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2995..3235 /label=SV40 3'UTR /ApEinfo_fwdcolor=#80ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3498..3498 /label=C to A polymorphysm /ApEinfo_fwdcolor=#ff8080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2316..2993 /label=DsRed /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2091..2296 /label=3XP3 enhancer/jsp70 promotor /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1835..1857 /label=New Feature /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(2051..2084) /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb complement(3242..3275) /label=LoxP(1) /ApEinfo_label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1708..1727 /label=HT82:CG31813 gDNA F#1 /ApEinfo_fwdcolor=#00ffff /ApEinfo_revcolor=#ff8040 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature complement(2198..2219) /label=OMS316 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2728..2747 /label=OMS317 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3574..3593 /label=HT85:CG31813 gDNA R#2 /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1835..1837 /label=PAM sequence /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1838..1857 /label=5' CRISPR targetting sequence /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3490..3492 /label=PAM sequence(1) /ApEinfo_label=PAM sequence /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3470..3489 /label=3' CRISPR targetting sequence /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 835..854 /label=5' homology Forward primer for attp /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4474..4495 /label=3' homology arm R primer for attp /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 672..692 /label=HT187:FWD /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4579..4599 /label=HT188:Rev /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2166..2189 /label=HT50:Rev /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 168..187 /label=HT195:FWD /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2034..2055 /label=HT26:Rev /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3156..3181 /label=HT31:FWD /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5089..5108 /label=HT196:Rev /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 catgactgtg gcacaaccca gaatccaatg ttttggcaac aaggcaccag aaaaaggcca 61 agcaactccc ccatattttt ggccttttgc tgctcggggc cattgtgagt tacccggaga 121 aagtcaaata acttaggcaa atccggaaag gccaaaagag ttttgtctca gctccgctgt 181 caggatgtgg cccccagcta gtgaatccac gtcttagagg agactcgaga ctgccatacg 241 gggatctgcg atcgaaaaaa gccatgttct gatgttcttc tttgataaat tttaattgtt 301 agtgtggtaa aaaaaaaggc aaccatgtac cgaaaaccta tgtagatcta acatatacct 361 atgatttgga taaattccaa aggaagaagt attacatata tttgggttat tttatttctt 421 tcatacttaa tggatctatc tttatatctg tttaagcatt ctccacttat ttgcaaatga 481 tgaacggctt ccgcttttac tttcagaata tggttgagct ttaaaaaaaa tgtatgaaac 541 aaatatatga taagagctga acgctttacg actgaagccc ttgtccactt agactattcc 601 cttcactgca tccatgacca tgaaatttcc agagatggct tctccctgga cgaagacaat 661 gcagttaatc ggaggaggaa gagcaggatt cgggatatcc aatgccagtc gtagggacat 721 gtgagccctc ttttgttggc tgaaattgaa aatcctttgt tcacccacta agcgcttttt 781 aattagattt caagcacgcc tgaataggtg tcctgcgaat cggacacaag gatctccccg 841 tagatgcatt gctcatgctc atgcctgtcg ttcagccttt aatcagatta aagtttctgg 901 agcgtttttt taacaactgc ttcttaggcc aaataaaaat caacaattgt aaatcatttg 961 aacaaatgtc gaccacgtga tttccggcgg cacgtgcttc agctccaagg cgccccatct 1021 tccggttccg ccaacagctg ccgaagcaga aattattggc aaagcgttta agcgcaataa 1081 atatgtttga ttgctggcaa gttgcccaaa aagatgtata tctaaagata aatatatacg 1141 taaagctgga cgggaatcgc tggcttccgg catcctgcga tcctttttca cccacaccag 1201 cagctccgaa ctcagagcaa atttgtaatt catttaaacg cgcaactaaa ccgcttcaag 1261 ctgtttgctc tgcacttttt aatagtcgca gattgtactc catactgaga tcactgtgac 1321 aaatgtaatg tattttgcaa aataaatata taggtattca aaaaaaaaat gttcttcatt 1381 tatctttata cgtagtacat attatatgta aaaaaatctt cgttttcttt aatacatttt 1441 tcaaaaatta atattcagtt taaataaagg ggcatggcat tctttttttt tgcagtgcag 1501 tttcaggaag ctgcacggac ggaactccgc tgaattatcc agccagccaa aggggccacg 1561 agcattgctc gactaaatca aacgaaagcg cagctaaaac gcttttgtgc aattcgatta 1621 tttacgttta ttcttttttt aaatgatatt tccagtctgg ccagtgggtt cggtggcacg 1681 aggagcaaga aaccaattat ttgccaacgg agcggagtag ctgttagcca gccggagggc 1741 aagcaatttg taaggaaatg aagcgcaagc aggggaaccg gaaaatgtgc tgcagttgaa 1801 aggcgaaagg gtaaacaaaa gatcaaatta atagccgcac agcatcgata tcgaaaccgg 1861 gttgattggt ggggggcaaa tacagctgtg tgtgttgcaa catcttgcag tgggcaaaca 1921 ttgaaacagg tgccatggct ttggagctcc agcggatcgg atgggcatag gtataaaaaa 1981 ccggaggtga cacctcgaaa GTAGTGCCCC AACTGGGGTA ACCTTTGAGT TCTCTCAGTT 2041 GGGGGCGTAG ATAACTTCGT ATAATGTATG CTATACGAAG TTATCGTACG GGATCTAATT 2101 CAATTAGAGA CTAATTCAAT TAGAGCTAAT TCAATTAGGA TCCAAGCTTA TCGATTTCGA 2161 ACCCTCGACC GCCGGAGTAT AAATAGAGGC GCTTCGTCTA CGGAGCGACA ATTCAATTCA 2221 AACAAGCAAA GTGAACACGT CGCTAAGCGA AAGCTAAGCA AATAAACAAG CGCAGCTGAA 2281 CAAGCTAAAC AATCGGCTCG AAGCCGGTCG CCACCATGGC CTCCTCCGAG GACGTCATCA 2341 AGGAGTTCAT GCGCTTCAAG GTGCGCATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA 2401 TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA 2461 CCAAGGGCGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCCCAGTTC CAGTACGGCT 2521 CCAAGGTGTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CAAGAAGCTG TCCTTCCCCG 2581 AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC 2641 AGGACTCCTC CCTCCAGGAC GGCTCCTTCA TCTACAAGGT GAAGTTCATC GGCGTGAACT 2701 TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACTATGGG CTGGGAGGCG TCCACCGAGC 2761 GCCTGTACCC CCGCGACGGC GTGCTGAAGG GCGAGATCCA CAAGGCCCTG AAGCTGAAGG 2821 ACGGCGGCCA CTACCTGGTG GAGTTCAAGT CCATCTACAT GGCCAAGAAG CCCGTGCAGC 2881 TGCCCGGCTA CTACTACGTG GACTCCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA 2941 CCATCGTGGA GCAGTACGAG CGCGCCGAGG GCCGCCACCA CCTGTTCCTG TAGGGGCCGC 3001 GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC 3061 TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT 3121 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 3181 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACCGG 3241 TATAACTTCG TATAATGTAT GCTATACGAA GTTATagata gttagtttta aatcggcctg 3301 ttttaaagaa gcacagtcta attttaaaca agtctaatgc ccttttcgtt aacaaatgtg 3361 taataataga catgattaag tttaatatgc aggtttttaa gctttaaagt agtacttaat 3421 atgtactcca ttacagaaaa atcattgtta aagagcctgt agaagtgccg gcaaactttc 3481 aacagcttct ggttaaacat ttgcaaagcg cagggcagtg tcggcctgat tcgcattctt 3541 tgcgacgtcc cgtcgtcctt tcgtaccatc gtcctttggt cctttggtcg tgtgccatcc 3601 ctgcagcgac gtgacatgtg acaagaagtt gccgcgatgt tgctgcaaca gctgctgtcg 3661 cctggcggct gaaatccttg gtccttcctg ctcgtgcgag cacccgaaaa atgatgaatg 3721 tgccaatgtt gctgggctgt tgccacatcc tcttggtctt ggtcttggtc ctggccgtgc 3781 ccctcataca ctctgccaaa cttccttggc tttgtgctgc agtcgtggcg aaagttttgc 3841 ggccgccgtc aagtttgtat ccattccgag actattagac accatttggt gcaacatgag 3901 ctgcccgtac ccgcggcggt acttggcggc agtgttaact ccagctggaa caccgccagt 3961 ccatcaagac agaagtttgt ccaagtgtag gactgcagcc gatctctgaa aacgtcaaat 4021 gaagcccaca aatagtttgc ggcctgcagg aaattaaact catatatgaa tggagtgcat 4081 cggccagtgc catgctgcac atcccacatg atgggaccct cataatgtca tcatcatcat 4141 cctccaggat ttggaattcg ggtaacacag ataatgagcc atgtatgtta cggcattggt 4201 ttttggttat cgtttaatta aggcagcccc atgcgaaatg tgcattgtga aatgtttgga 4261 agtttgtaat ttcctctgca gctgcaacat gtgtccatat tcattggcat tcactaagcg 4321 acaccgagat gtagacaaaa agtttggcct ttgtctgggt tgattcagcc aacaataggc 4381 ggccattgtt tggcttttag cagcaacaaa agccaaagag ccaaacaatt tagataggac 4441 aataagccaa cggaaattaa attggctgct ctagtttaca tagtagttct cgcccatcat 4501 ctcgaacaag aaagcaaaga aagctgtttt gaaacgaact taaattaaac tttcaaacga 4561 atgatatatg tacaaacggt gagagtcgga atgaaagcat aattattatt actcatccat 4621 acaccggtct catttataat ctgcctgtgt ttgataagct tagaactggc aaaattaatt 4681 taaattggaa tttactctga gaaaatttaa agtagtggct ttaatttcca attgagctct 4741 tagattatac cttcattcat ttgcacttca tctcaaaacc aatgtacaat gctaaaaaaa 4801 aaatacacaa aacaacagct tgaaacttgg tatttcttgt tcaatgcaat tgaaaccgcc 4861 gaaaaagaaa ctgagaatat tattcaataa ttttaatatt ccacaatggt tctcagctcg 4921 attgccttcc ttgaatggga cttaatacaa atccgctgaa caagatgaaa acaaaaatct 4981 gttattcaga ttgacttggc acaaaagccg acctcggcca aaaagcttgt acatatatat 5041 gtatgtattc agtttttcct tgctatatta aatacttttt cgagcccact gttactttcg 5101 gtgcgggaca acaagaataa cgagcaaatt ttcgcagcga gggggcagaa atatggaaaa 5161 tcacttgccg attttcaatt gagcggcgga aatgtaacgg aaataaattt acataaatac 5221 aatatggtgc gtgcttcccg gcgccatttt atttgcacaa cacatacgca gctct //