LOCUS       pMGXn-Flp        9572 bp DNA     circular SYN 12-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    136..996
                     /label=AmpR
                     /note="AmpR"
     rep_origin      1151..1770
                     /direction=RIGHT
                     /label=pBR322_ori
                     /note="pBR322_ori"
     misc_feature    1817..2101
                     /label=attB
                     /note="attB"
     misc_feature    2122..6249
                     /label=miniwhite
                     /note="miniwhite"
     promoter        6504..7711
                     /label=nosP
                     /note="nosP"
     CDS             7715..8989
                     /label=FlpG5D Variant
                     /note="FlpG5D Variant"
     terminator      8990..9497
                     /label=bam3'UTR+Synthetic PolyA
                     /note="bam3'UTR+Synthetic PolyA"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcagatctt ccttgatctc cttgttctta tccatctccg
     6541 ccttcatgtt gtcgaagaac tgcgagaaga agccggccct gcgacctggc gcactataaa
     6601 agcggggctg cggagtaaag tcatgactta ggccggttgc gccatggggt tttatgagcc
     6661 aaactctaac ctgctgctgc tgcaggttct gcgaggcctg ctggcacgtg aagagacagg
     6721 cgcgatcccg cacaagggca gctattctgt actgcaagga gaaaaaatca ttgaaagctt
     6781 cgaccgtttt aacctcgaaa tatgcacatg taaggacgga tgtgagcgaa cgccagtgat
     6841 gaccgggatc agaggtaacc taccatggtg gggattaggt gaccgttcgc aggtagtttg
     6901 atcggagcga atgttcgggg ggtctggcgt cagaggctct aaactttatg taattcctgc
     6961 cgcgaaacac gcacgtatca agcagtcagc tgttctcttc gttcagcgcg cgccggtgtt
     7021 gcaaaacgag cgctcttcgc cggcggtggc tcgtgcgata gttcgttttg tcggtaatcc
     7081 gatgttgccg cgccgatatc atgtgatgtt gtcacagtgc gcgaaattcg aatggtggtg
     7141 tgcagtgatt gtgttgtgac ggcgagtggc gcgtgtgggt gcttagtttt gggagatgtt
     7201 ttcgtatttt tttgttgata actcaggctt tgttgctgtg ttgtagtact attttccatt
     7261 gcgcggtgtc cagcttttaa ttagtggcac atattcttag caagtaaaaa ttattttgca
     7321 tactattaaa tttcttataa attattttct aaaattaagt ttaccttttc aattttacta
     7381 aaaatatcga tatatttatt atcgctggaa aactacatta ttccacctct aagcaagaac
     7441 cgttagttgg cgcgtagctt taccacaaaa ttcctggaat tgccgtacgc ttcgcagttg
     7501 tttcaagttg tctaagggac atacgatttt ttttgcctct gcgtcacgat tttaacccaa
     7561 aagcgagttt agttacatgt acattattat tagataaaga agtatcgcga atacttcagt
     7621 tgaataaact gtgcttggtt tttgggtgag gatttgtgga aagtagagtg cgcgataacc
     7681 gtaactttcg acccggattt tcgccggtac caatatgccg cagtttgata tcctctgcaa
     7741 gaccccacca aaggtgttgg tgcgtcaatt cgtggagcga tttgagaggc cgtcgggtga
     7801 gaagatcgcc ctgtgcgctg ccgagttgac ctatttgtgt tggatgatca ctcataatgg
     7861 caccgcgatt aagcgcgcta cctttatgag ctataacact atcattagca attccctgtc
     7921 ctttgacata gtaaacaagt ccctgcagtt taaatacaag actcagaagg ccactatatt
     7981 ggaggcttcg ctgaaaaagt tgatcccggc atgggagttc acgatcatcc catactacgg
     8041 tcagaaacac cagagcgata ttaccgatat tgtaagcagc ctccagctgc agtttgagtc
     8101 cagcgaagag gctgataagg gtaatagtca cagcaaaaag atgctgaagg cactgctgtc
     8161 cgagggcgaa agcatctggg agattactga gaaaatcctg aactcgtttg agtacaccag
     8221 ccgattcacc aagacgaaga ccctgtacca gttcctcttt ttggcaacct tcatcaattg
     8281 tggtcgcttc agtgacatca aaaacgtgga ccctaaatcg ttcaagctgg tgcagaataa
     8341 gtacctgggt gttatcatcc agtgcttggt taccgagact aagacctccg tgtccaggca
     8401 catttatttc ttctccgcaa ggggtcgcat cgatccgctg gtatacttgg atgagttcct
     8461 tcggaatagt gaaccagtct tgaagcgcgt gaacaggacg ggcaattcca gtagcaacaa
     8521 gcaagagtac cagctgctga aggataatct tgttcggtcg tacaacaaag ccttgaagaa
     8581 aaatgcgcca tactcgatct ttgcaatcaa gaatggtccc aagagtcata ttggccggca
     8641 cctgatgacc agtttcttgt ccatgaaggg tctgactgag ctcacaaacg tcgttggcaa
     8701 ctggtcggac aagcgagcga gtgccgtcgc ccgcacgaca tacacccacc agatcacggc
     8761 cataccagat cactatttcg cgctggtgtc gcgttattat gcctatgatc ccatcagcaa
     8821 ggagatgatc gcgctgaagg acgaaaccaa tccaatcgag gagtggcagc atatcgagca
     8881 acttaaggga agcgctgagg gtagcatccg ttaccccgcc tggaacggca tcatcagcca
     8941 ggaggttctg gattacctga gctcctacat caatcgccgt atttaatagc tagcactaat
     9001 gctgtgcaca tcgataaaag aatgacagca aatatgcaat ttaaaaaagc tactcttctc
     9061 atgggaagca ataatttcgt aaagtaaaca tatctatagt gtaagatata tttgtccaat
     9121 agtgcggact ccatatttgt attcgtgaat aagcttatat aagcttttta aaaatattta
     9181 tcaaatcgat acaaacaaaa tcaaaatgaa aacgatttat tacccctgtt ttgagattga
     9241 taacaaattt atataagttt aactgtgttt acatttattt ggcaaaacta caaatgtgtt
     9301 tgcttttcac ttttataaat ctgtatttta cttaaacttt agaaataaga aatcctttag
     9361 tgcctgaatt tattttgcaa ctacgtttta tttgtatgag gaacttacca gtttttctta
     9421 tttgctttgc atttgtattt tgaaagtcaa ataaatactc tttattttca tttgatctgt
     9481 gtgttggttt tttgtgtgaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     9541 taggttaatg tcatgataat aatggtttct ta
//
